Dual signal amplification coupling with DNA-templated silver nanoclusters for sensitive and label-free detection of thrombin

Abstract Sensitive and reliable determination of thrombin is relevant in the realms medical biological research as it serves an essential biomarker a number blood-related illnesses. Herein, we integrate allosteric probe-based specific identification dual signal amplification to present unique fluorescent technique for label-free sensitive detection. Based on DNA polymerase endonuclease-assisted amplification, method exhibits high sensitivity with low limit detection 2.3 pM, while maintaining excellent selectivity stability. More importantly, approach successfully applied analyzing effect nalbuphine coagulation function mice. Overall, this possesses advantages specificity thrombin, which promising diagnosis diseases.